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Addgene

pJL_h2b
(Plasmid #239174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239174 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone size w/o insert (bp) 3239
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    h2b (Smp_108390)
  • Alt name
    Smp_108390
  • Species
    Schistosoma mansoni
  • Insert Size (bp)
    360

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning Primers: Forward-GCCTCCTAAAGTTGTGTCTGG, Reverse-GACCCACTGTACTTCGTCACTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJL_h2b was a gift from Phillip Newmark (Addgene plasmid # 239174 ; http://n2t.net/addgene:239174 ; RRID:Addgene_239174)

  • For your References section:

    The esophageal gland mediates host immune evasion by the human parasite Schistosoma mansoni. Lee J, Chong T, Newmark PA. Proc Natl Acad Sci U S A. 2020 Aug 11;117(32):19299-19309. doi: 10.1073/pnas.2006553117. Epub 2020 Jul 31. 10.1073/pnas.2006553117 PubMed 32737161
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