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Addgene

pJC-lecg
(Plasmid #239180)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239180 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Vector type
    T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lecg
  • Alt name
    SMED30019646
  • Species
    Schmidtea mediterranea

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eam1105I (destroyed during cloning)
  • 3′ cloning site Eam1105I (destroyed during cloning)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single-stranded probes can be generated with SP6 or T3 RNA polymerases

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC-lecg was a gift from Phillip Newmark (Addgene plasmid # 239180 ; http://n2t.net/addgene:239180 ; RRID:Addgene_239180)

  • For your References section:

    Somatic regulation of female germ cell regeneration and development in planarians. Khan UW, Newmark PA. Cell Rep. 2022 Mar 15;38(11):110525. doi: 10.1016/j.celrep.2022.110525. 10.1016/j.celrep.2022.110525 PubMed 35294875
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