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Addgene

Sc Pol3-FLAG(Ec)-pRSF405/GAL
(Plasmid #239198)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239198 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS405/GAL
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pol3
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    E. coli codon bias (synthetic gene), but same amino acid sequence as wild type
  • Entrez Gene
    POL3 (a.k.a. YDL102W, CDC2, HPR6, TEX1)
  • Tag / Fusion Protein
    • 3X FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc Pol3-FLAG(Ec)-pRSF405/GAL was a gift from Michael O'Donnell (Addgene plasmid # 239198 ; http://n2t.net/addgene:239198 ; RRID:Addgene_239198)
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