Sc [Pol31 + Pol32]-pRS403/GAL
(Plasmid
#239199)
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PurposeOvererxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS4034/GAL
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePol31
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenePOL31 (a.k.a. YJR006W, HUS2, HYS2, SDP5)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namePol32
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenePOL32 (a.k.a. YJR043C, REV5)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sc [Pol31 + Pol32]-pRS403/GAL was a gift from Michael O'Donnell (Addgene plasmid # 239199 ; http://n2t.net/addgene:239199 ; RRID:Addgene_239199)