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Sc RFC[hk-1s + 5]-pLANT/RIL
(Plasmid #239200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLANT/RIL
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RFC1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    N-terminal truncation
  • Entrez Gene
    RFC1 (a.k.a. YOR217W, CDC44)
  • Tag / Fusion Protein
    • His-10 + PKA kinase motif on truncated Sc RFC1 (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RFC5
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RFC5 (a.k.a. YBR087W)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

"RIL" indicates that tRNAs for rare yeast (S. cerevisiae) codons for amino acids R, I, & L are encoded in the plasmid for better expression of S. cerevisiae genes in E. coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Sc RFC[hk-1s + 5]-pLANT/RIL was a gift from Michael O'Donnell (Addgene plasmid # 239200 ; http://n2t.net/addgene:239200 ; RRID:Addgene_239200)
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