Sc RFC[2+3+4]-pET
(Plasmid
#239203)
-
PurposeOverexpress Sc RFC2, RFC3, & RFC4 in E. coli
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 239203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET11a
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert 1
-
Gene/Insert nameRFC2
-
SpeciesS. cerevisiae (budding yeast)
-
Entrez GeneRFC2 (a.k.a. YJR068W)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameRFC3
-
SpeciesS. cerevisiae (budding yeast)
-
Entrez GeneRFC3 (a.k.a. YNL290W)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Gene/Insert 3
-
Gene/Insert nameRFC4
-
SpeciesS. cerevisiae (budding yeast)
-
Entrez GeneRFC4 (a.k.a. YOL094C)
Cloning Information for Gene/Insert 3
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Sc RFC[2+3+4]-pET was a gift from Michael O'Donnell (Addgene plasmid # 239203 ; http://n2t.net/addgene:239203 ; RRID:Addgene_239203)