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pLJC6-HK1 (delta MBD)-3xFLAG
(Plasmid #239253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239253 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLJC6
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HK1
  • Species
    H. sapiens (human)
  • Mutation
    Insert contains the set of silent mutations described for pLJC5-HK1-3xFLAG (See comments for further detail)
  • Entrez Gene
    HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase)
  • Promoter Ubc
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GTAAATTGTCCGCTAAATTCTGGC
  • 3′ sequencing primer CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The nucleotides corresponding to the N-terminal 21 amino acids were replaced with a new initiator methionine and GGS linker to remove the endogenous mitochondrial binding domain (MBD)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJC6-HK1 (delta MBD)-3xFLAG was a gift from Jason Cantor (Addgene plasmid # 239253 ; http://n2t.net/addgene:239253 ; RRID:Addgene_239253)

  • For your References section:

    Hexokinase detachment from mitochondria drives the Warburg effect to support compartmentalized ATP production. Huggler KS, Flickinger KM, Forsberg MH, Mellado Fritz CA, Chang GR, McGuire MF, Capitini CM, Cantor JR. Nat Metab. 2026 Jan 16. doi: 10.1038/s42255-025-01428-1. 10.1038/s42255-025-01428-1 PubMed 41545565
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