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pLentiCRISPR-v1-sgHK2_14
(Plasmid #239257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239257 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLentiCRISPR-v1
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA_14 targeting HK2
  • gRNA/shRNA sequence
    TAGCCAGGCATTCGGCAATG
  • Species
    H. sapiens (human)
  • Entrez Gene
    HK2 (a.k.a. HKII, HXK2)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer GTTTTAAAATGGACTATCATATGCTTACCG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiCRISPR-v1-sgHK2_14 was a gift from Jason Cantor (Addgene plasmid # 239257 ; http://n2t.net/addgene:239257 ; RRID:Addgene_239257)

  • For your References section:

    Hexokinase detachment from mitochondria drives the Warburg effect to support compartmentalized ATP production. Huggler KS, Flickinger KM, Forsberg MH, Mellado Fritz CA, Chang GR, McGuire MF, Capitini CM, Cantor JR. Nat Metab. 2026 Jan 16. doi: 10.1038/s42255-025-01428-1. 10.1038/s42255-025-01428-1 PubMed 41545565
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