LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583)
(Plasmid
#239260)
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PurposeLentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RMRP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneLentiCRISPR-B
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Vector typeLentiviral, CRISPR
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameU6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RMRP
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gRNA/shRNA sequencesgRNA1: GAGGACGTGGTTCGTGCTGA/sgRNA2: GCTCAGCGGGATACGCTTCT
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CGTGACGTAGAAAGTAATAATTTCTTGGGTAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.01.19.633795 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LentiCRISPR-B_2xsgRNAs-RMRP (pAVA3583) was a gift from Andrei Alexandrov (Addgene plasmid # 239260 ; http://n2t.net/addgene:239260 ; RRID:Addgene_239260) -
For your References section:
Identification of RMP24 and RMP64, human ribonuclease MRP-specific protein components. Che R, Mirani B, Panah M, Chen X, Luo H, Alexandrov A. Cell Rep. 2025 Jun 24;44(6):115752. doi: 10.1016/j.celrep.2025.115752. Epub 2025 May 24. 10.1016/j.celrep.2025.115752 PubMed 40413743