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Addgene

pSLQ10625
(Plasmid #239268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239268 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAGGS vector backbone
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-dPspCas13b-EGFP-ABI
  • Species
    Prevotella sp. P5-125
  • Insert Size (bp)
    7060
  • Mutation
    H133A and H1058A in PspCas13b
  • Promoter CAG
  • Tags / Fusion Proteins
    • Flag tag (N terminal on insert)
    • V5 tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLQ10625 was a gift from Stanley Qi (Addgene plasmid # 239268 ; http://n2t.net/addgene:239268 ; RRID:Addgene_239268)

  • For your References section:

    Programmable control of spatial transcriptome in live cells and neurons. Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, Qi LS. Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z. Epub 2025 May 21. 10.1038/s41586-025-09020-z PubMed 40399675
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