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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426
(Plasmid #239294)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239294 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Backbone size w/o insert (bp) 5726
  • Total vector size (bp) 5851
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    T7 promoter
  • Insert Size (bp)
    39

Gene/Insert 2

  • Gene/Insert name
    ITS-tRNAIle intron
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    66

Gene/Insert 3

  • Gene/Insert name
    T7 terminator - Var1
  • Insert Size (bp)
    14

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The T7 promoter-ITS-tRNAIle intron-T7 terminator (Var1) sequence is inserted between SalI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7pro-tRNA-ITSIle intron-T7tVar1-pRS426 was a gift from Anita Hopper (Addgene plasmid # 239294 ; http://n2t.net/addgene:239294 ; RRID:Addgene_239294)

  • For your References section:

    Free introns of tRNAs as complementarity-dependent regulators of gene expression. Nostramo RT, Sinopoli PL, Bao A, Metcalf S, Peltier LM, Hopper AK. Mol Cell. 2025 Feb 20;85(4):726-741.e6. doi: 10.1016/j.molcel.2025.01.019. Epub 2025 Feb 11. 10.1016/j.molcel.2025.01.019 PubMed 39938518
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