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Addgene

pDCX_mEmerald_CRISPR_HDR
(Plasmid #239331)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239331 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2800
  • Total vector size (bp) 4455
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEmerald and DCX homology arms
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1763
  • Promoter None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDCX_mEmerald_CRISPR_HDR was a gift from Torsten Wittmann (Addgene plasmid # 239331 ; http://n2t.net/addgene:239331 ; RRID:Addgene_239331)
  • For your References section:

    Doublecortin reinforces microtubules to promote growth cone advance in soft environments. Dema A, Charafeddine RA, van Haren J, Rahgozar S, Viola G, Jacobs KA, Kutys ML, Wittmann T. Curr Biol. 2024 Nov 21:S0960-9822(24)01458-1. doi: 10.1016/j.cub.2024.10.063. 10.1016/j.cub.2024.10.063 PubMed 39626666