pDCX_mEmerald_CRISPR_HDR
(Plasmid
#239331)
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PurposeHDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2800
- Total vector size (bp) 4455
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEmerald and DCX homology arms
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1763
- Promoter None
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDCX_mEmerald_CRISPR_HDR was a gift from Torsten Wittmann (Addgene plasmid # 239331 ; http://n2t.net/addgene:239331 ; RRID:Addgene_239331) -
For your References section:
Doublecortin reinforces microtubules to promote growth cone advance in soft environments. Dema A, Charafeddine RA, van Haren J, Rahgozar S, Viola G, Jacobs KA, Kutys ML, Wittmann T. Curr Biol. 2024 Nov 21:S0960-9822(24)01458-1. doi: 10.1016/j.cub.2024.10.063. 10.1016/j.cub.2024.10.063 PubMed 39626666