pmStayGold_I3
(Plasmid
#239336)
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PurposeExpresses the I3-01 nanocage subunit N-terminally tagged with mStayGold in mammalian cells. Assembles into nanocages tagged with 60 FPs.
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 239336-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 239336-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5357
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameI3-01
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Alt nameI3 nanocage subunit
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SpeciesSynthetic
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Insert Size (bp)609
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MutationK129A
- Promoter CMV
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Tag
/ Fusion Protein
- mStayGold (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 239336-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 239336-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmStayGold_I3 was a gift from Torsten Wittmann (Addgene plasmid # 239336 ; http://n2t.net/addgene:239336 ; RRID:Addgene_239336) -
For your References section:
Quantitative comparison of fluorescent proteins using protein nanocages in live cells. Viola G, Ibrahim YW, Jacobs KA, Lemiere J, Kutys ML, Wittmann T. J Cell Sci. 2025 May 15;138(10):jcs263858. doi: 10.1242/jcs.263858. Epub 2025 May 21. 10.1242/jcs.263858 PubMed 40326462
