Mirror RFP[ENE(WT)-mascRNA](pAVA2987)
(Plasmid
#239348)
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PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239348 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneFireworks RFP[PTC-] (AVA2515)
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Vector typeMammalian Expression
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Selectable markersATG-lacking-HygroR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMALAT1 ENE(WT)-mascRNA
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SpeciesH. sapiens (human)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.26.615073 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mirror RFP[ENE(WT)-mascRNA](pAVA2987) was a gift from Andrei Alexandrov (Addgene plasmid # 239348 ; http://n2t.net/addgene:239348 ; RRID:Addgene_239348) -
For your References section:
Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach. Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A. Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3. 10.1038/s41467-025-59998-3 PubMed 40399278