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Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874)
(Plasmid #239353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239353 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Fireworks GFP[PTC-] (AVA2598)
  • Vector type
    Mammalian Expression
  • Selectable markers
    ATG-lacking-PuroR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MALAT1 ENE(WT)-mascRNA(mut 8356-8370)
  • Species
    H. sapiens (human)
  • Mutation
    MALAT1 ENE(WT)-mascRNA(mut 8356-8370: ctacgaccaccaacc)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.26.615073 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mirror GFP[ENE(WT)-mascRNA(mut 8356-8370)](pAVA3874) was a gift from Andrei Alexandrov (Addgene plasmid # 239353 ; http://n2t.net/addgene:239353 ; RRID:Addgene_239353)

  • For your References section:

    Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach. Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A. Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3. 10.1038/s41467-025-59998-3 PubMed 40399278
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