PKA1280
(Plasmid
#239370)
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PurposeHis-Las17 (300-422) pTOPO 6xHis-Las17 (300-422) as a BamH1-HindIII fragment
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 239370 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepTOPO 6xHis
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth TemperatureRoom Temperature
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Growth Strain(s)DH5alpha
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Growth instructionsOvernight 17°C
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLas17 (amino acids 300-422)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)360
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GenBank IDYOR181W
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Tag
/ Fusion Protein
- 6xHis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKA1280 was a gift from Kathryn Ayscough (Addgene plasmid # 239370 ; http://n2t.net/addgene:239370 ; RRID:Addgene_239370)