PKA1317
(Plasmid
#239371)
-
PurposeS.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 239371 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGEX6P1
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5400
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameSLA1 (5-131)
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)400
-
MutationW108A
-
GenBank IDYBL007C
-
Tag
/ Fusion Protein
- GST
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PKA1317 was a gift from Kathryn Ayscough (Addgene plasmid # 239371 ; http://n2t.net/addgene:239371 ; RRID:Addgene_239371)