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Addgene

PKA1336
(Plasmid #239372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX6P1
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5350
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Overnight 17°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Las17 (amino acids 300-422)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    360
  • Mutation
    Arginines 319,322 both to alanine
  • GenBank ID
    YOR181W
  • Tag / Fusion Protein
    • GST

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PKA1336 was a gift from Kathryn Ayscough (Addgene plasmid # 239372 ; http://n2t.net/addgene:239372 ; RRID:Addgene_239372)
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