PKA1252
(Plasmid
#239373)
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PurposeGST-Sec4 (full length) cloned in BamH1 and Sal1 sites of pGEX-6P1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239373 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepGEX6P1
- Backbone size w/o insert (bp) 4995
- Total vector size (bp) 5650
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSec4
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)645
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GenBank IDYFL005W
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Entrez GeneSEC4 (a.k.a. YFL005W, SRO6)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PKA1252 was a gift from Kathryn Ayscough (Addgene plasmid # 239373 ; http://n2t.net/addgene:239373 ; RRID:Addgene_239373)