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Addgene

D10A-Cas9
(Plasmid #239383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239383 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV-PE2 (Addgene #132775)
  • Backbone manufacturer
    David Liu
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9-D10A
  • Species
    S. pyogenes
  • Mutation
    D10A in Cas9
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • BPNLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D10A-Cas9 was a gift from Caixia Gao (Addgene plasmid # 239383 ; http://n2t.net/addgene:239383 ; RRID:Addgene_239383)

  • For your References section:

    Circular RNA-mediated inverse prime editing in human cells. Liang R, Wang S, Cai Y, Li Z, Li KM, Wei J, Sun C, Zhu H, Chen K, Gao C. Nat Commun. 2025 May 31;16(1):5057. doi: 10.1038/s41467-025-59120-7. 10.1038/s41467-025-59120-7 PubMed 40447589
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