D10A-Cas9
(Plasmid
#239383)
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PurposeFor circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239383 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCMV-PE2 (Addgene #132775)
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Backbone manufacturerDavid Liu
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenCas9-D10A
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SpeciesS. pyogenes
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MutationD10A in Cas9
- Promoter CMV
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on insert)
- BPNLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
D10A-Cas9 was a gift from Caixia Gao (Addgene plasmid # 239383 ; http://n2t.net/addgene:239383 ; RRID:Addgene_239383)