iPEmax-D10A
(Plasmid
#239386)
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PurposeFor inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239386 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCMV-PEmax (Addgene #174820)
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Backbone manufacturerDavid Liu
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenCas9-D10A-R221K-N394K, M-MLV-RT
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SpeciesS. pyogenes, MMLV
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MutationD10A, R221K, N394K in Cas9
- Promoter CMV
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Tags
/ Fusion Proteins
- BPNLS (N terminal on insert)
- SV40 NLS, c-myc NLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iPEmax-D10A was a gift from Caixia Gao (Addgene plasmid # 239386 ; http://n2t.net/addgene:239386 ; RRID:Addgene_239386) -
For your References section:
Circular RNA-mediated inverse prime editing in human cells. Liang R, Wang S, Cai Y, Li Z, Li KM, Wei J, Sun C, Zhu H, Chen K, Gao C. Nat Commun. 2025 May 31;16(1):5057. doi: 10.1038/s41467-025-59120-7. 10.1038/s41467-025-59120-7 PubMed 40447589