pSec61β-mScarlet3-H (aka mYongHong)
(Plasmid
#239414)
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PurposeExpresses Sec61b-mScarlet3-H in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec61β
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SpeciesH. sapiens (human)
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MutationM163H in mScarlet3
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Entrez GeneSEC61B
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet3-H (aka mYongHong) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSec61β-mScarlet3-H (aka mYongHong) was a gift from Zhifei Fu (Addgene plasmid # 239414 ; http://n2t.net/addgene:239414 ; RRID:Addgene_239414) -
For your References section:
A highly stable monomeric red fluorescent protein for advanced microscopy. Xiong H, Chang Q, Ding J, Wang S, Zhang W, Li Y, Wu Y, Lin P, Yang C, Liu M, Fang G, Yang Y, Xie J, Qi D, Jiang T, Fu W, Hu F, Chen Y, Yue R, Li Y, Cui Y, Li M, Fan S, Yang Y, Xu Y, Li D, Zhang F, Zhao H, Wu C, Zheng Q, Piatkevich KD, Fu Z. Nat Methods. 2025 Apr 17. doi: 10.1038/s41592-025-02676-5. 10.1038/s41592-025-02676-5 PubMed 40247125