U6-sgRNA-BbsI-CMV-EGFP cloning vector
(Plasmid
#239464)
-
Purpose(Empty Backbone) sgRNA cloning vector with EGFP expression cassette. An sgRNA spacer sequence can be cloned into BbsI sites. A CMV-EGFP expression cassette is included as a reporter of transfection efficiency.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepMA-T
- Backbone size (bp) 2380
-
Vector typeMammalian Expression, CRISPR
- Promoter U6 and CMV
-
Selectable markersEGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byThe CMV promoter and EGFP gene sequence were cloned from pCMV-GFP (Connie Cepko, Addgene #11153) into the gRNA cloning Vector Bbs I ver. 2 (Hodaka Fujii, Addgene #85586)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
U6-sgRNA-BbsI-CMV-EGFP cloning vector was a gift from Patrick Harrison (Addgene plasmid # 239464 ; http://n2t.net/addgene:239464 ; RRID:Addgene_239464)
Map uploaded by the depositor.
