p1300-dCas9-HV5
(Plasmid
#239479)
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PurposeAn intermediate vector is used to assemble three gRNAs via Golden Gate cloning.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCAMBIA1300
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Vector typePlant Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBQ-dCAS9
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p1300-dCas9-HV5 was a gift from Jian-Kang Zhu (Addgene plasmid # 239479 ; http://n2t.net/addgene:239479 ; RRID:Addgene_239479) -
For your References section:
Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis. He L, Yao Y, You Y, Wei X, Ma Y, Yuan W, Lang Z, Zhu JK. Nat Commun. 2025 Dec 6. doi: 10.1038/s41467-025-66933-z. 10.1038/s41467-025-66933-z PubMed 41353342