pPSG803
(Plasmid
#239533)
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PurposeCRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTMP136
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Backbone manufacturerInternal
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Vector typeCRISPR
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA_YMR206W
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SpeciesSynthetic
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional insert: Cas9
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSG803 was a gift from John Dueber (Addgene plasmid # 239533 ; http://n2t.net/addgene:239533 ; RRID:Addgene_239533)