Skip to main content
Addgene

pVL1708
(Plasmid #239542)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239542 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    TMP138
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional inserts: 1) GEM; 2) TEV protease; 3) ScPex22(1-36)-mRuby2-SH3pep

Please note: This plasmid contains a single nucleotide deletion that adds six amino acids to C-terminus of mRuby. This difference does not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVL1708 was a gift from John Dueber (Addgene plasmid # 239542 ; http://n2t.net/addgene:239542 ; RRID:Addgene_239542)
Related items: