pKS1614
(Plasmid
#239545)
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PurposeExpress truncated VoGES, Idi1, and Erg20WW for synthesis of geraniol in the peroxisome with Venus as peroxisomal marker
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneTMP138
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Backbone manufacturerInternal
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametVoGES-ePTS1
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SpeciesS. cerevisiae (budding yeast); Valeriana officinalis
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional inserts: 1) ScIdi1p-ePTS1; 2)ScErg20p(F96W_N127W)-ePTS1; 3) Venus-ePTS1
Please note: This plasmid contains a different terminator than ScADH1 Terminator. This difference does not impact plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKS1614 was a gift from John Dueber (Addgene plasmid # 239545 ; http://n2t.net/addgene:239545 ; RRID:Addgene_239545)