pFUW mTagBFP2-P2A-NeoR
(Plasmid
#239568)
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PurposeCytosolic expression of mTagBFP2
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239568 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFUW
- Backbone size w/o insert (bp) 9221
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Vector typeLentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2
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SpeciesEntacmaea quadricolor
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Insert Size (bp)1593
- Promoter UbC
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFUW mTagBFP2-P2A-NeoR was a gift from Tim Ahfeldt & Joel Blanchard (Addgene plasmid # 239568 ; http://n2t.net/addgene:239568 ; RRID:Addgene_239568)