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Addgene

pET30a-SSDH
(Plasmid #239573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Backbone size w/o insert (bp) 5408
  • Total vector size (bp) 6854
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can use XL-1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Succinate-semialdehyde dehydrogenase
  • Alt name
    SSDH
  • Alt name
    gabD
  • Species
    E. coli
  • Insert Size (bp)
    1446
  • Entrez Gene
    gabD (a.k.a. b2661, ECK2655)
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-SSDH was a gift from William Plaxton (Addgene plasmid # 239573 ; http://n2t.net/addgene:239573 ; RRID:Addgene_239573)

  • For your References section:

    Cost-effective production of Escherichia coli ‘GABase’ for spectrophotometric determination of γ-aminobutyrate (GABA) levels or glutamate decarboxylase activity. Benidickson KH, Symonds KF, Snedden WA, Plaxton WC. Biology Methods and Protocols, 2025. doi: 10.1093/biomethods/bpaf050 10.1093/biomethods/bpaf050
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