pXYB083
(Plasmid
#239579)
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PurposePlant RNA Vision constructs with 82 bp gRNAs together with RFP input
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239579 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAMBIA
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Vector typePlant Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namesfGFPn_Y66-P1_loop_01-stem01-gRNA03
Gene/Insert 2
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Gene/Insert namegRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
Gene/Insert 3
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Gene/Insert nameRFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXYB083 was a gift from Xiaohan Yang (Addgene plasmid # 239579 ; http://n2t.net/addgene:239579 ; RRID:Addgene_239579) -
For your References section:
A split ribozyme system for in vivo plant RNA imaging and genetic engineering. Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X. Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 10.1111/pbi.14612. Epub 2025 Feb 7. 10.1111/pbi.14612 PubMed 39919021