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Addgene

CaMK2rep-S603A
(Plasmid #239623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5481
  • Total vector size (bp) 6661
  • Modifications to backbone
    EM7 promoter-BleoR instead of NeoR/KanR
  • Vector type
    Mammalian Expression
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpotNES-sGFP2-SynP3-3xmyc
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    1236
  • Mutation
    changed Serine 603 to Alanine
  • GenBank ID
    NM_019133.2
  • Entrez Gene
    Syn1
  • Promoter CMV
  • Tags / Fusion Proteins
    • Spot (N terminal on insert)
    • sGFP2 (N terminal on insert)
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTTGACTCACGGGGATTTCCAAGTC
  • 3′ sequencing primer CCCACCCCACCCCCCAGAATAGAATGACA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.
Depositor confirms P89S in sGFP2, changes are not expected to impact function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CaMK2rep-S603A was a gift from F Javier Díez Guerra (Addgene plasmid # 239623 ; http://n2t.net/addgene:239623 ; RRID:Addgene_239623)
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