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Addgene

prhaBAD-anti-rabbit-Nb-Hia5
(Plasmid #239627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239627 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pD861
  • Backbone manufacturer
    ATUM Bio
  • Backbone size w/o insert (bp) 2260
  • Total vector size (bp) 4664
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hia5
  • Alt name
    DNA Adenine Methyltransferase
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    843
  • Mutation
    Codon optimized for bacterial expression
  • GenBank ID
    JF268249.1
  • Promoter rhaBAD
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • TEV (N terminal on insert)
    • anti-Rabbit Nanobody (N terminal on insert)
    • 6HIS (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For a protocol for the purification of the proteins, please visit https://www.protocols.io/view/purification-and-activity-testing-for-nanobody-hia-6qpvrqbyzlmk/v1.

Please visit https://doi.org/10.1101/2025.07.01.662447 for bioRxiv preprint.

Plasmid also referenced in https://doi.org/10.1101/2025.03.11.642717.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prhaBAD-anti-rabbit-Nb-Hia5 was a gift from Aaron Straight (Addgene plasmid # 239627 ; http://n2t.net/addgene:239627 ; RRID:Addgene_239627)

  • For your References section:

    Histone H3 lysine methyltransferase activities control compartmentalization of human centromeres. Sidhwani P, Schwartz JP, Fryer KA, Straight AF. bioRxiv 2025.07.01.662447; doi: https://doi.org/10.1101/2025.07.01.662447 10.1101/2025.07.01.662447
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