pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH
(Plasmid
#239748)
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PurposePlasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepShip
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRuby2
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SpeciesEntacmaea quadricolor
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.26.625483 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH was a gift from Kate Galloway (Addgene plasmid # 239748 ; http://n2t.net/addgene:239748 ; RRID:Addgene_239748) -
For your References section:
High-resolution profiling reveals coupled transcriptional and translational regulation of transgenes. Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE. Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528 10.1093/nar/gkaf528 PubMed 39651241