pcDNA3.1(+)_Nav1.6WT-HA
(Plasmid
#239762)
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PurposeExpresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.
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Depositing Lab
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Depositing OrganizationUniversitaetsklinikum Tuebingen
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 239762-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 239762-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)-P2A-eGFP (P2A-eGFP was replaced by the HA tag)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)XL10 Gold
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Growth instructionsGrowth temperature: 27-28 °C. All the steps involving propagation of Nav1.6 in bacteria were performed at 27-28 °C to avoid the introduction of additional mutations and rearrangements of Scn8a (details are provided in Stajkovic et al., 2023).
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameScn8a
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SpeciesM. musculus (mouse)
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Entrez GeneScn8a (a.k.a. C630029C19Rik, NaCh6, Nav1.6, dmu, med, mnd-2, mnd2, nmf2, nmf335, nmf58, nur14, seal)
- Promoter CMV
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Tag
/ Fusion Protein
- HA-tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The gene encoding Nav1.6 was synthesized by GenScript.
DNA (Catalog # 239762-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 239762-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)_Nav1.6WT-HA was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 239762 ; http://n2t.net/addgene:239762 ; RRID:Addgene_239762) -
For your References section:
Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry. Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche H, Nikic-Spiegel I. J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. Epub 2023 Jun 28. 10.1242/jcs.260600 PubMed 37288813
