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pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA
(Plasmid #239766)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239766 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)-P2A-eGFP (P2A-eGFP was replaced by the HA tag)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    Growth temperature: 27-28 °C. All the steps involving propagation of Nav1.6 in bacteria were performed at 27-28 °C to avoid the introduction of additional mutations and rearrangements of Scn8a (details are provided in Stajkovic et al., 2023).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scn8a
  • Species
    M. musculus (mouse)
  • Mutation
    K1425TAG, Y371C
  • Entrez Gene
    Scn8a (a.k.a. C630029C19Rik, NaCh6, Nav1.6, dmu, med, mnd-2, mnd2, nmf2, nmf335, nmf58, nur14, seal)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A portion of this plasmid was derived from a plasmid made by GenScript.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 239766 ; http://n2t.net/addgene:239766 ; RRID:Addgene_239766)

  • For your References section:

    Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry. Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche H, Nikic-Spiegel I. J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. Epub 2023 Jun 28. 10.1242/jcs.260600 PubMed 37288813
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