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Addgene

pALN148
(Plasmid #239800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMJ28 (pBABE Hygro mEGFP)
  • Backbone size w/o insert (bp) 6410
  • Total vector size (bp) 7952
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mm-CENPT-Rn-Histone Fold Domain-4NS
  • Species
    M. musculus (mouse), R. norvegicus (rat)
  • Insert Size (bp)
    1568
  • Mutation
    4 nucleotide substitution in rat histone fold domain 1. T1454A (L to Q), 2. GA1408-1409TT (E to L), 3. C1388T (T to I), 4. g1294A (A to T)
  • Promoter Moloney Murine Leukemia Virus (MMLV) LTR promoter
  • Tag / Fusion Protein
    • mEGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XHOI (unknown if destroyed)
  • 3′ cloning site ECORI (unknown if destroyed)
  • 5′ sequencing primer EGFP-C-Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pALN148 was a gift from Iain Cheeseman & Ally Nguyen (Addgene plasmid # 239800 ; http://n2t.net/addgene:239800 ; RRID:Addgene_239800)

  • For your References section:

    Adaptive evolution of CENP-T modulates centromere binding. Dudka D, Nguyen AL, Boese KG, Marescal O, Akins RB, Black BE, Cheeseman IM, Lampson MA. Curr Biol. 2025 Mar 10;35(5):1012-1022.e5. doi: 10.1016/j.cub.2025.01.017. Epub 2025 Feb 12. 10.1016/j.cub.2025.01.017 PubMed 39947176
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