pGY225
(Plasmid
#239809)
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PurposeExpress H3-mRFP1 in filamentous fungi
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepFC332
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Vector typeFungal Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH3
- Promoter Tef1IN
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Tag
/ Fusion Protein
- mRFP1
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGY225 was a gift from Kyle Pomraning (Addgene plasmid # 239809 ; http://n2t.net/addgene:239809 ; RRID:Addgene_239809) -
For your References section:
Strong Translation Elongation Factor 1-Alpha (tef1α) Promoter Reporter Systems for Aspergillus niger. Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, Shuang Deng, Beth A. Hofstad, and Kyle R. Pomraning. ACS Synthetic Biology Article ASAP 10.1021/acssynbio.5c00368