pGY232
(Plasmid
#239810)
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PurposeExpress EGFP-P2A-mRFP1 in filamentous fungi
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239810 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepFC332
- Total vector size (bp) -2
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Vector typeFungal Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEGFP
- Promoter Tef1IN
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Tag
/ Fusion Protein
- 2A
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemRFP1
- Promoter Tef1IN
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGY232 was a gift from Kyle Pomraning (Addgene plasmid # 239810 ; http://n2t.net/addgene:239810 ; RRID:Addgene_239810) -
For your References section:
Strong Translation Elongation Factor 1-Alpha (tef1α) Promoter Reporter Systems for Aspergillus niger. Guoliang Yuan, Tatiana Serrano-Zayas, Ziyu Dai, Erica I. DeMers, Shuang Deng, Beth A. Hofstad, and Kyle R. Pomraning. ACS Synthetic Biology Article ASAP 10.1021/acssynbio.5c00368
Map uploaded by the depositor.
