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pT3TS-RfxCas13d-2C-NLS
(Plasmid #239816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239816 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pT3TS
  • Backbone size w/o insert (bp) 3118
  • Total vector size (bp) 6115
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rfx-Cas13d
  • Alt name
    CasRx
  • Species
    Ruminococcus flavefaciens XPD3002
  • Insert Size (bp)
    2997
  • Tags / Fusion Proteins
    • SV40 NLS (C terminal on insert)
    • Nucleoplasmin long NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is owned by Moreno-Mateos Lab (CABD, Seville, Spain)

Please note: This plasmid contains proline as the third amino acid of nucleoplasmin NLS. This is a known variant of nucleoplasmin NLS that does not impact its function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT3TS-RfxCas13d-2C-NLS was a gift from Miguel Angel Moreno-Mateos (Addgene plasmid # 239816 ; http://n2t.net/addgene:239816 ; RRID:Addgene_239816)

  • For your References section:

    Enhanced RNA-targeting CRISPR-Cas technology in zebrafish. Moreno-Sanchez I, Hernandez-Huertas L, Nahon-Cano D, Martinez-Garcia PM, Treichel AJ, Gomez-Marin C, Tomas-Gallardo L, da Silva Pescador G, Kushawah G, Egidy R, Perera A, Diaz-Moscoso A, Cano-Ruiz A, Walker JA 2nd, Munoz MJ, Holden K, Galceran J, Nieto MA, Bazzini AA, Moreno-Mateos MA. Nat Commun. 2025 Mar 16;16(1):2591. doi: 10.1038/s41467-025-57792-9. 10.1038/s41467-025-57792-9 PubMed 40091120
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