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pHLsec-FAP
(Plasmid #239817)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239817 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHLsec
  • Backbone size w/o insert (bp) 4511
  • Total vector size (bp) 6987
  • Modifications to backbone
    None
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fibroblast activation protein alpha
  • Alt name
    Seprase
  • Alt name
    FAP
  • Alt name
    Fibroblast activation protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2229
  • Mutation
    No mutations
  • GenBank ID
    NM_004460.5 NM_004460.5
  • Entrez Gene
    FAP (a.k.a. DPPIV, FAPA, FAPalpha, SIMP)
  • Promoter Chicken beta-actin
  • Tags / Fusion Proteins
    • 8xHIS tag (C terminal on insert)
    • TwinStrep tag (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Not known, cloned by GenScript
  • 3′ sequencing primer Not known, cloned by GenScript
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLsec-FAP was a gift from Ingrid De Meester (Addgene plasmid # 239817 ; http://n2t.net/addgene:239817 ; RRID:Addgene_239817)

  • For your References section:

    In vitro kinetic profiling by enzymatic approaches to select FAPIs with a long residence time. Verhulst E, Brzeminski P, de Groot A, Wals JW, Michiels R, Fabisiak A, Thys J, Grintsevich S, Van Rymenant Y, Peeters S, Lambregts S, Sim Y, Verstraete R, Van Wielendaele P, Berg M, Elvas F, Van Ostade X, Rosch F, Lambeir AM, De Winter H, Van Der Veken P, De Meester I. Eur J Med Chem. 2026 Feb 15;304:118494. doi: 10.1016/j.ejmech.2025.118494. Epub 2025 Dec 18. 10.1016/j.ejmech.2025.118494 PubMed 41455278
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