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Addgene

pHS2096
(Plasmid #239835)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239835 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET45b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NovaIscB
  • Species
    Human gut metagenome assembly
  • Mutation
    Insertion of REC domain from NbaCas9-1 and swap of two loops in the REC domain, resulting in engineered IscB
  • Promoter T7
  • Tag / Fusion Protein
    • 14xHis-TwinStrep-bdSUMO (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS2096 was a gift from Feng Zhang (Addgene plasmid # 239835 ; http://n2t.net/addgene:239835 ; RRID:Addgene_239835)

  • For your References section:

    Evolution-guided protein design of IscB for persistent epigenome editing in vivo. Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure G, Moeller L, Pham J, Mears KS, Ni HM, Macrae RK, Zhang F. Nat Biotechnol. 2025 May 7. doi: 10.1038/s41587-025-02655-3. 10.1038/s41587-025-02655-3 PubMed 40335752
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