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12mer-FKBP
(Plasmid #239852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239852 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 6342
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    12mer-FKBP
  • Species
    Synthetic
  • Insert Size (bp)
    1113
  • Promoter T7
  • Tag / Fusion Protein
    • 6X His Tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    12mer-FKBP was a gift from Cole DeForest (Addgene plasmid # 239852 ; http://n2t.net/addgene:239852 ; RRID:Addgene_239852)

  • For your References section:

    Stimuli-triggered formation of de novo-designed protein biomaterials. Gregorio NE, Li Z, Hoye JW, David Baker D, DeForest CA. Cell Biomaterials, 2025 10.1016/j.celbio.2025.100239
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