pLJ54 (Pmyo-2_GCaMP8f_SL2_mKate2_let-858_3'UTR)
(Plasmid #239864)

• Purpose: expression of GCaMP8f in C. elegans under myo2-promoter control
• Depositing Lab: Monika Scholz
• Publication: Liu et al Genetics. 2024 Oct 7;228(2):iyae125. doi: 10.1093/genetics/iyae125.

Backbone

• Vector backbone: pRL231 (Punc-31_GCaMP7f_unc-54 3’UTR)
• Backbone manufacturer: gift from Dr. Manuel Zimmer, University of Vienna
• Backbone size w/o insert (bp): 2550
• Total vector size (bp): 6593
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: GCaMP8f
• Species: Synthetic
• Insert Size (bp): 1500
• Mutation: codon optimized with introns for C. elegans
• Promoter: myo-2
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe I (not destroyed)
• 3′ cloning site: Kpn I (not destroyed)
• 5′ sequencing primer: oLJ297F
• 3′ sequencing primer: oLJ298R

Gene/Insert 2

• Gene/Insert name: mKate2
• Species: Synthetic
• Mutation: codon optimized with introns for C. elegans
• Promoter: myo-2

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Apa I (not destroyed)
• 5′ sequencing primer: oLJ304F
• 3′ sequencing primer: oLJ323R

Resource Information

• Supplemental Documents:
  • pLJ54_myo-2p_GCaMP8f_SL2 mKate2 let-858_3'UTR.gb
• A portion of this plasmid was derived from a plasmid made by: derived from cDNA described in Zhang Y, Rózsa M, Liang Y, Bushey D, et al. 2023. "Fast and sensitive GCaMP calcium indicators for imaging neural populations." Nature. 615:884–891. doi:10.1038/s41586-023-05828-9.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry