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LLP1014
(Plasmid #239936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239936 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCK2
  • Vector type
    Plant Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp18.2::sgRNA-B
  • Species
    Other
  • Mutation
    N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: This plasmid contains an IS4 transposable element inserted in the backbone. This insertion does not impact plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP1014 was a gift from Ryan Lister (Addgene plasmid # 239936 ; http://n2t.net/addgene:239936 ; RRID:Addgene_239936)

  • For your References section:

    CRISPRi-based circuits to control gene expression in plants. Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, Secco D, Small I, Kidd BN, Lister R. Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 10.1038/s41587-024-02236-w. Epub 2024 May 20. 10.1038/s41587-024-02236-w PubMed 38769424
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