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LLP1019
(Plasmid #239941)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239941 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Binary
  • Vector type
    Plant Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaMV 35S::dCas9-ZAT10(1x)
  • Alt name
    Other genes: TCTP::Csy4 and 35S::sgRNA-A
  • Species
    Other
  • Mutation
    N/A

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LLP1019 was a gift from Ryan Lister (Addgene plasmid # 239941 ; http://n2t.net/addgene:239941 ; RRID:Addgene_239941)

  • For your References section:

    CRISPRi-based circuits to control gene expression in plants. Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, Secco D, Small I, Kidd BN, Lister R. Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 10.1038/s41587-024-02236-w. Epub 2024 May 20. 10.1038/s41587-024-02236-w PubMed 38769424
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