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Addgene

pYL116
(Plasmid #239967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239967 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pJS496
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PuroR-T2A-mCherry with homology arms for human Suz12
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYL116 was a gift from Thomas Cech (Addgene plasmid # 239967 ; http://n2t.net/addgene:239967 ; RRID:Addgene_239967)

  • For your References section:

    Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools. Long Y, Cech TR. Methods. 2021 Jul;191:44-58. doi: 10.1016/j.ymeth.2021.01.002. Epub 2021 Jan 12. 10.1016/j.ymeth.2021.01.002 PubMed 33444739
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