pYL117
(Plasmid
#239968)
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PurposeCRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJS496
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Vector typeCRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYL117 was a gift from Thomas Cech (Addgene plasmid # 239968 ; http://n2t.net/addgene:239968 ; RRID:Addgene_239968) -
For your References section:
Targeted mutagenesis in human iPSCs using CRISPR genome-editing tools. Long Y, Cech TR. Methods. 2021 Jul;191:44-58. doi: 10.1016/j.ymeth.2021.01.002. Epub 2021 Jan 12. 10.1016/j.ymeth.2021.01.002 PubMed 33444739