pTol2-stmn1b-KALTA4
(Plasmid
#239987)
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PurposeExpresses GAL4 under control of the stmn1b promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTol2-myl7(cmlc2):GFP
- Total vector size (bp) 13061
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Vector typeZebrafish Tol2/phiC31 compatible
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameKALTA4
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Alt nameGAL4
- Promoter stmn1b
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer stmn1b (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameEGFP
- Promoter myl7
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer myl7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2-stmn1b-KALTA4 was a gift from Summer Thyme (Addgene plasmid # 239987 ; http://n2t.net/addgene:239987 ; RRID:Addgene_239987) -
For your References section:
Genetic context of transgene insertion can influence neurodevelopment in zebrafish. Moyer AJ, Barcus A, Capps MES, Chrabasz JA, Lalonde RL, Mosimann C, Thyme SB. bioRxiv [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 10.1101/2025.02.28.640904. 10.1101/2025.02.28.640904 PubMed 40093151