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Addgene

6xHsa.NFKB:d2EGFP
(Plasmid #239990)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239990 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNFkB:EGFP
  • Backbone manufacturer
    John Rawls (Addgene plasmid #44922)
  • Backbone size w/o insert (bp) 6534
  • Total vector size (bp) 7380
  • Modifications to backbone
    The eGFP from pNFkB:EGFP backbone was excised using ClaI (R0197S, NEB) and NcoI-HF (R3193S, NEB) following the manufacturers’ instructions.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Destabilized EGFP
  • Alt name
    d2EGFP
  • Insert Size (bp)
    846
  • Promoter cfos minimal promoter

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer tccaccggtcgccaccATGGTGAGCAAGG GCGAG
  • 3′ sequencing primer catgtctggatcatcatcgatCTACACATTGA TCCTAGCAGAAGC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gene was amplified from a plasmid by PCR and subcloned into pNFkB backbone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHsa.NFKB:d2EGFP was a gift from Raquel Espin-Palazon (Addgene plasmid # 239990 ; http://n2t.net/addgene:239990 ; RRID:Addgene_239990)
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