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pAAV-CMV-DIO-SaCas9-U6-sgDaglb
(Plasmid #240023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240023 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PX601-AAV-CMV::NLS-SaCas9-NLS- 3xHA-bGHpA;U6::BsaI-sgRNA
  • Total vector size (bp) 7481
  • Vector type
    Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    diacylglycerol lipase, beta
  • Alt name
    E330036I19Rik
  • gRNA/shRNA sequence
    CCCATCATTGACCAGTCTGCG
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_144915
  • Entrez Gene
    Daglb (a.k.a. E330036I19Rik)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknow (unknown if destroyed)
  • 3′ cloning site Unknow (unknown if destroyed)
  • 5′ sequencing primer U6F, LKO-15
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-DIO-SaCas9-U6-sgDaglb was a gift from Huaibin Cai (Addgene plasmid # 240023 ; http://n2t.net/addgene:240023 ; RRID:Addgene_240023)

  • For your References section:

    Deficiency in endocannabinoid synthase DAGLB contributes to early onset Parkinsonism and murine nigral dopaminergic neuron dysfunction. Liu Z, Yang N, Dong J, Tian W, Chang L, Ma J, Guo J, Tan J, Dong A, He K, Zhou J, Cinar R, Wu J, Salinas AG, Sun L, Kumar M, Sullivan BT, Oldham BB, Pitz V, Makarious MB, Ding J, Kung J, Xie C, Hawes SL, Wang L, Wang T, Chan P, Zhang Z, Le W, Chen S, Lovinger DM, Blauwendraat C, Singleton AB, Cui G, Li Y, Cai H, Tang B. Nat Commun. 2022 Jun 17;13(1):3490. doi: 10.1038/s41467-022-31168-9. 10.1038/s41467-022-31168-9 PubMed 35715418
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